Beilstein J. Org. Chem.2010,6, 732–741, doi:10.3762/bjoc.6.87
; heterobifunctionalcross-linker; hydrolysis; kinetics; thiophosphate; Introduction
Heterobifunctional cross-linking agents are used widely in protein science for forming covalently-bonded protein-protein complexes [1] and protein-small molecule systems [2]. S-Alkylation and N-acylation processes are used together
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Graphical Abstract
Scheme 1:
Use of 2-bromoacetic acid esters as heterobifunctional cross-linking agents.